POLRMT

Size

£655.00

SKU:: 590620
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to 11aa near the C-terminal of human POLRMT, located within the last 50aa of POLRMT. Species sequence homology: bovine (82%); Chimpanzee (100%) ; monkey (82%); feline (90%)
Format:: Supplied as a liquid in PBS, 0.02% sodium azide.
Extra Details:: This gene encodes a mitochondrial DNA-directed RNA polymerase. The gene product is responsible for mitochondrial gene expression as well as for providing RNA primers for initiation of replication of the mitochondrial genome. Although this polypeptide has the same function as the three nuclear DNA-directed RNA polymerases, it is more closely related to RNA polymerases of phage and mitochondrial polymerases of lower eukaryotes. TFB2M and POLRMT are essential for mammalian mitochondrial DNA replication. POLRMT mutations impair mitochondrial transcription causing neurological disease. Knock-down of MRPL12 by RNA interference results in instability of POLRMT. Applications: Suitable for use in ELISA, Western Blot and Immunofluorescence. Other applications not tested. Recommended Dilutions: Western Blot: 2ug/ml Immunofluorescence: 10ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice