Vesicular Stomatitis Virus (VSV-G) tag

Size

£722.00

SKU:: 590532
Purity:: Purified by ammonium sulfate precipitation.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Vesicular stomatitis virus (VSV-G) tag (YTDIEMNRLGK) coupled to GST.
Format:: Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Extra Details:: Epitope tagging is a widely accepted technique that fuses an epitope peptide to a certain protein as a marker for gene expression. With this technique, gene expression can be easily monitored on Western blotting, immunoprecipitation and immunofluorescence utilizing an antibody that recognizes such an epitope. The VSV-G Tag (YTDIEMNRLGK) corresponds to the partial peptide sequence of the vesicular stomatitis virus glycoprotein. Applications: Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:100, Colorimetric Immunocytochemistry: 10ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.