TRAIL

Size

£514.00

SKU:: 590485
Application:: Flow Cytometry
Purity:: Purified by affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Human TRAIL transfected mouse cell line.
Clone:: RIK-2
Format:: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Extra Details:: Induces apoptosis in tumorigenic and transformed cell lines, involved in T cell mediated cytotoxicity, TRAIL ligand of cognate receptors induces NF-kappa-B signaling in a variety of cell types. TRAIL is a 30kD protein known as TNF-related apoptosis inducing ligand, CD253, TNFSF10, or Apo-2L. It can be expressed as a cell surface (30kD) and soluble ligand (19-20kD) produced by megakaryocytes and platelets, monocytes, neutrophils, NK cells and activated T cells. This antigen is also expressed on tumor cells and transformed cell lines. TRAIL has been reported to induce apoptosis in tumor and transformed cell lines by a caspase-dependent process. Applications: Suitable for use in Flow Cytometry. Other applications have not been tested. Recommended Dilutions: Flow Cytometry: ≤ 1ug/ml/10e6 cells in 100ul Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.