590454X
RNA Polymerase II (Ascites Free)
Size
£895.00
- SKU:
- 590454X
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Linear peptide corresponding to human RNA polymerase II.
- Clone:
- CTD4H8
- Format:
- Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide,. (Ascites Free)
- Extra Details:
- RNA polymerase II (Pol II) is a multi-subunit enzyme responsible for the transcription of protein-coding genes. Transcription initiation requires recruitment of the complete transcription machinery to a promoter via solicitation by activators and chromatin remodeling factors. Pol II can coordinate 10 to 14 subunits. This complex interacts with the promoter regions of genes and a variety of elements and transcription factors. The DNA binding domain of the polymerase is a groove where TFIIB orients the DNA for unwinding and transcription. Applications: Suitable for use in Immunocytochemistry, Western Blot and ChIP. Other applications not tested. Recommended Dilution: Western Blot: 0.05-1ug/ml detected RNA polymerase II in HeLa nuclear extracts. Immunocytochemistry: 1:50 and 1:250 dilution fdetected RNA polymerase II in HeLa and A431 cells, respectively. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice