PIP2

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£728.00

SKU:: 590436
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Liposomes containing dimyristoyl phosphatidylcholine, cholesterol, PIP2 from bovine spinal cord, and lipid A. Species Sequence Homology: bovine
Clone:: KT10
Format:: Supplied as a liquid in 0.1 M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Extra Details:: PI(4,5)P2 (PIP2) is synthesized mainly by the phosphorylation of PI by PI 4- kinases that produce phosphatidylinositol 4-phosphate (PI4P), which is then phosphorylated by PI4P 5-kinase (PIP5K). Some PIP2 can also be generated through dephosphorylation of PI(3,4,5)P3 by PTEN. Phosphoryl transfer to positions 4 and 5 in PIP2 requires ATP and the cofactor Mg2+. Although most protein kinases achieve maximal active state at low-micromolar intracellular concentrations of ATP, lipid kinases generally require much higher concentrations of ATP to support their activity. Following its synthesis, PIP2 modulates the functions of various proteins including actin-regulating proteins and ion channels. It also serves as a substrate for phospholipase C (PLC) and a source for second messengers, inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG) that are involved in activation and membrane localization of protein kinase C and in the modulation of ion channel activity in endothelial cells and smooth muscle cells. G-protein (Gq) activation that can result in rapid activation of PLC, can reduce PIP2 levels in cells within seconds. The hydrolysis of PIP2 is shown to be enhanced in cells transformed by oncogenes and tumor viruses. Clone KT10 binds to PIP2 even at picogram levels; however, it does not react with PIP or PI even at microgram levels. It is shown to inhibit the proliferation of Src- and erbB-transformed cells but do not affect the proliferation of untransformed cells. It is also reported to abolish PDGF-induced mitogenesis in both untransformed and ras-transformed cells but does not affect the basal level of mitogenesis. Applications: Suitable for use in ELISA, Flow Cytometry, Inhibition assay and Immunocytochemistry. Other applications not tested. Recommended Dilution: Immunocytochemistry: 1:100 detected PI(4,5)P2 (PIP2) in K562 cells. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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