590428
PDGF Receptor Beta
Size
£812.00
- SKU:
- 590428
- Purity:
- Purified by Protein G affinity chromatography from hybridoma tissue culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresonding to human PDGF Receptor beta.
- Clone:
- 42G12
- Format:
- Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol.
- Extra Details:
- Mitogenic platelet derived growth factor (PDGF) signaling is mediated by the PDGF tyrosine kinase receptors PDGFRα and PDGFRbeta, which exist as homo- or heterodimer isoforms (alpha alpha, beta beta, alphabeta). Secretion of PDGFs and expression of PDGFRs in a broad distribution of cell types contributes to regulation of cellular processes including cell growth, actin reorganization, prevention of apoptosis, and chemotaxis. Structurally, the transmembrane PDGFRs are composed of an N-terminal extracellular domain with five immunoglobulin repeats, and an intracellular domain containing two spaced tyrosine kinase domains. PDGF ligands display preferential affinity to PDGFRalpha and PDGFRbeta with PDGFRbeta activation restricted to binding by PDGF BB and PDGF DD. Growth factor binding initiates receptor dimerization and activates the cytosolic tyrosine kinase domains. Applications: Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence and electron microscopy. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml, ECL Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice