590415
p53
Size
£800.00
- SKU:
- 590415
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to human p53.
- Clone:
- TP53/1799R
- Format:
- Supplied as a liquid in PBS, 0.1mg/ml BSA, 0.05% sodium azide. Also available without BSA or azide, see 590415X.
- Extra Details:
- p53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis. It binds to MDM2, SV40 T antigen and human papilloma virus E6 protein. Positive nuclear staining with p53 antibody has been reported to be a negative prognostic factor in breast carcinoma, lung carcinoma, colorectal, and urothelial carcinoma. Anti-p53 positivity has also been used to differentiate uterine serous carcinoma from endometrioid carcinoma as well as to detect intratubular germ cell neoplasia. Mutations involving p53 are found in a wide variety of malignant tumors, including breast, ovarian, bladder, colon, lung, and melanoma. Applications: Suitable for use in Western Blot, Immunohistochemistry and Flow Cytometry. Other applications not tested. Recommended Dilution: Western Blot: 1-2g/ml Immunofluorescence: 0.5-1ug/ml Immunohistochemistry (formalin-fixed paraffin sections): 1-2ug/ml for 30 min at RT. Requires HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min. Flow Cytometry: 1-2ug/10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice