Nucleolin

Size

£819.00

SKU:: 590408
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Human Nucleolin from Raji cell extract.
Clone:: 4E2
Format:: .Supplied as a liquid in PBS, 50% glycerol.
Extra Details:: Nucleolin (identical to human DNA helicase IV) is an abundant, non-ribosomal protein of the nucleolus involved in the organization of nuclear chromatin, rDNA transcription, packaging of pre-RNA, ribosome assembly, nucleocytoplasmic transport, cytokinesis, nucleogenesis, and apoptosis. A predominantly nuclear protein, the presence of nucleolin at the surface of cancer cells suggests that it could be valuable as a marker for the diagnosis of cancer. Applications: Suitable for use in Western Blot, Immunoprecipitation, immunohistochemistry, Immunocytochemistry and Flow Cytometry. Other applications not tested. Recommended Dilutions: Western Blot (ECL): 1ug/ml Immunohistochemistry: 1-10ug/ml Immunocytochemistry: 1-10ug/ml Flow Cytometry: 5-10ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.