NKX2.2

Size

£774.00

SKU:: 590396
Application:: IHC-Fr
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Partial recombinant protein corresponding to human NKX2.2.
Clone:: RMNK2-1 (recomb)
Format:: Supplied as a liquid in PBS, 0.1mg/ml BSA, 0.05% sodium azide.
Extra Details:: NKX2.2 acts as a transcriptional activator. Required for the maintenance of NEUROD1 expression in the horomone-producing endocrine cells of the pancreas. May be involved in specifying diencephalic neuromeric boundaries, and in controlling the expression of genes that play a role in axonal guidance. Associates with chromatin at the NEUROD1 promoter region. Binds to a subset of consensus elements within the NEUROD1 promoter (By similarity) Applications: Suitable for use in Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry (formalin-fixed paraffin sections): 1-2ug/ml for 30 min at RT. Requires HIER: boil tissue sections in pH 9.0 10mM Tris, 1mM EDTA for 10-20 min followed by cooling at RT for 20 min. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.