590352
LAG-3
Size
£819.00
- SKU:
- 590352
- Purity:
- Purified from tissue culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Synthetic peptide corresponding to 30aa, GPPAAAPGHPLAPGPHPAAPSSWGPRPRRY from the first N-terminal D1 domain of human LAG-3.
- Clone:
- L4-PL33
- Format:
- Supplied as a liquid in PBS, 0.02% sodium azide, 10% glycerol.
- Extra Details:
- The lymphocyte activation gene-3 (LAG-3, CD223), a member of the immunoglobulin superfamily (IgSF) related to CD4, binds to the major histocompatibility complex (MHC) class II molecules but with higher affinity than CD4. Several alternative mRNA splice-variants of human LAG-3 have been described, two of them encoding potential secreted forms: LAG-3V1 (i.e. the D1-D2 domains of the protein, 36kD) and LAG-3V3 (D1-D3, 52kD). The longer form was detected by ELISA in the serum of healthy individuals as well as of tuberculosis patients with a favorable outcome. LAG-3 expression by T cell clones correlated with IFN-γ production, and hence soluble LAG-3 has been suggested as a serological marker of Th1 responses. Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry and Flow Cytometry. Recommended Dilution: Western Blot: 5ug/ml Immunoprecipitation: 10ug/ml Immunohistochemistry (FFPE): 1:150 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice