EpCAM

Size

£800.00

SKU:: 590169
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Full length recombinant protein corresponding to human EpCAM.
Clone:: EGP40/1555R (recomb)
Format:: Supplied as a liquid in PBS, 0.1mg/ml BSA, 0.05% sodium azide.
Extra Details:: EpCAM is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. It is also useful in distinguishing serous carcinomas of the ovary from mesothelioma. This epithelial antigen plays an important role as a tumor-cell marker in lymph nodes from patients with esophageal carcinoma otherwise classified as node-negative. Epithelial antigen has also been suggested as a discriminator between basal cell and baso-squamous carcinomas, and squamous cell carcinoma of the skin. Applications: Suitable for use in Immunohistochemistry, Flow Cytometry and Immunofluorescence. Other applications not tested. Recommended Dilution: Immunofluorescence: 1-2ug/ml Immunohistochemistry (formalin-fixed paraffin sections): 1-2ug/ml for 30 min at RT. Requires HIER. Flow Cytometry: 1-2ug/10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice