590153
DOG1
Size
£965.00
- SKU:
- 590153
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to full length human DOG1.
- Clone:
- DG1/1487R
- Format:
- Supplied as a liquid in 1X PBS, 0.1mg/ml BSA, 0.05% sodium azide.
- Extra Details:
- Expression of DOG-1 protein is elevated in the gastrointestinal stromal tumors (GISTs), c-kit signaling-driven mesenchymal tumors of the GI tract. DOG-1 is rarely expressed in other soft tissue tumors, which, due to appearance, may be difficult to diagnose. Immunoreactivity for DOG-1 has been reported in 97.8 % of scorable GISTs, including all c-kit negative GISTs. Overexpression of DOG-1 has been suggested to aid in the identification of GISTs, including platelet-derived growth factor Receptor alpha mutants that fail to express c-kit antigen. The overall sensitivity of DOG1 and c-kit in GISTs is nearly identical: 94.4% vs. 94.7%. Applications: Suitable for use in Immunohistochemistry, Flow Cytometry and Immunofluorescence. Other applications not tested. Recommended Dilution: Flow Cytometry: 0.5-1ug/106 cells in 0.1 ml Immunofluorescence: 0.5-1ug/ml Immunohistochemistry (formalin-fixed paraffin sections): 0.25-0.5ug/ml for 30 min at RT Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice