590131
Cyclin D1
Size
£633.00
- SKU:
- 590131
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to human cyclin D1.
- Clone:
- 5D4
- Format:
- Supplied as a liquid in PBS, pH 7.2, 50% glycerol.
- Extra Details:
- Cyclins are a family of proteins which function as the regulatory subunits of cyclin/cyclin-dependent kinase (Cdk) holoenzymes, mediating entry into and progression through the cell cycle. Each cyclin is expressed in a temporal fashion during the cell cycle, partnering with a specific Cdk to activate its protein kinase activity. The most well defined mitotic cyclins are: cyclin D, which partners with cdks 4 and 6 during early G1 phase; cyclin E, which activates Cdk2 in the G1 to S phase transition; cyclin A, which utilizes Cdks 1 and 2 during progression through S phase and into G2; and cyclin B, which activates Cdk1 in M-phase promotion. Applications: Suitable for use in Western Blot, Immunoprecipitation, immunohistochemistry, Immunocytochemistry and Flow Cytometry. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml, ECL Immunohistochemistry (frozen/paraffin): 10ug/ml Immunoprecipitation: 1-10ug/200ug cell extract from 5x106 cells Flow Cytometry: 10ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice