CD31

Size

£863.00

SKU:: 590071
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Full length recombinant protein corresponding to human CD31.
Clone:: C31/1395R (recomb)
Format:: Supplied as a liquid in PBS, 0.1mg/ml BSA, 0.05% sodium azide.
Extra Details:: CD31 is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. It is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. Applications: Suitable for use in Immunohistochemistry, Flow Cytometry and Western Blot. Other applications not tested. Recommended Dilution: Immunohistochemistry (formalin-fixed paraffin sections): 1-2ug/ml for 30 min at RT. Requires HIER: boil tissue sections in pH 9.0 10mM Tris, 1mM EDTA for 10-20 min followed by cooling at RT for 20 min. Flow Cytometry: 1-2ug/10e6 cells. Western Blot: 1-2ug/ml. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.