Caspase-8

Size

£741.00

SKU:: 590030
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to aa176-460 from human Caspase-8 at C-terminal.
Clone:: 5F7
Format:: Supplied as a liquid in PBS, pH 7.2, 50% glycerol.
Extra Details:: Caspase-8/FADD-like ICE (FLICE), is an ~55kD cytosolic protein belonging to the Fas/APO-1/CD95 death-inducing signaling complex. Although researchers have defined eight different isoforms of caspase-8 at the mRNA level (8/a-h), significant expression of only Caspase-8/a and /b occurs at the protein level in a variety of cell lines. Caspase-8 contains two death effector domains (DEDs) and exhibits sequence homology to both FADD, a signal transducer of Fas-induced apoptosis, and to the ICE/CED-3 family of cysteine proteases. Caspase-8 binds to FADD through its amino terminal DEDs and induces apoptosis when overexpressed. Activation of Caspase-8 involves a two-step proteolysis whereby the cleavage of Caspase-8 generates a 43kD fragment (p43) and a 12kD fragment further processed to 10kD, then the cleavage of receptor-associated p43 yields p26 and the release of the active site containing p18. A number of cytosolic factors inhibit the FADD-Caspase-8 containing signaling complex, including the ICE/CED-3 family inhibitor CrmA which inhibits Caspase-8 protease activity, and the short and long forms of FLIP that bind to the FADD-Caspase-8 complex. Applications: Suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot (ECL): 1ug/ml Immunoprecipitation: 1:50 Immunohistochemistry: paraffin sections Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice