589971
Beta-Catenin
Size
£652.00
- SKU:
- 589971
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to human Beta-Catenin (N-terminal fragment).
- Clone:
- 12F7
- Format:
- Supplied as a liquid in PBS, pH 7.2, 50% glycerol.
- Extra Details:
- Beta-Catenin, a vertebrate homologue of Drosophila armadillo encoded by the CTNNB1 gene, performs two functions in the cell: mediating cell-cell adhesion by association between E-cadherin and the cytoskeleton, and acting as a downstream transcriptional activator of Wnt signaling, a key player in such functions as embryonic development, the induction of cell polarity, and the determination of cell fate. In the absence of Wnt signaling, glycogen synthase kinase-3beta (GSK-3beta) phosphorylates any free β-catenin at the amino terminus. This results in adenomatous polyposis coli tumor suppressor protein (APC)-mediated beta-catenin degradation via the ubiquitin-proteosome pathway. Wnt signaling stabilizes beta-catenin by inhibiting the activity of GSK-3beta and preventing beta-catenin degradation. Beta-catenin forms complexes with DNA-binding proteins such as T-cell factor (Tcf) and lymphocyte-enhancer factor (Lef), which promote expression of a variety of target genes, including c-myc and cyclin D1. Applications: Suitable for use in Western Blot, Immunoprecipitation and immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml, ECL Immunohistochemistry: 1-5ug/ml Immunoprecipitation: 2ug/200-300ul cell extract Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice