589961
AU1 (DTYRYI) Epitope Tag
Size
£744.00
- SKU:
- 589961
- Purity:
- Purified by affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Peptide corresponding to DTYRYI (the same epitope as the monoclonal AU1 antibody).
- Format:
- Supplied as a liquid in PBS, 0.03% thimerosal.
- Extra Details:
- AU1 is a commonly used epitope that is tagged onto the N- or C- terminus of a protein of interest so that the tagged protein can be analyzed and visualized. Antibodies against AU1 epitope recognize proteins containing AU1 epitope tag fused to targeted proteins and allow for specific detection in several immunochemical assays such as western blotting, ELISAs and immunostaining techniques. This antibody may be preferred over the monoclonal AU1 for experiments in murine systems or in experiments involving two proteins labeled with two different tags. Because of the small size of the epitope, it is unlikely to alter the activity of the cloned sequence. Applications: Suitable for use in ELISA, Western Blot, Immunocytochemistry and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000-1:30,000 Immunocytochemistry: 1:200-1:2000 ELISA: 1:100-1:400 (coating), 1:1000-1:30,000 (primary) Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice