Alpha-Catenin

Size

£984.00

SKU:: 589945
Purity:: Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to human alpha-catenin.
Clone:: 15D9
Format:: Supplied as a lyophilized powder in 1ml of 2x PBS, 0.09% sodium azide PEG, and sucrose. Reconstitute with 1ml sterile dH2O.
Extra Details:: Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Immunocytochemistry: (0.1-1ug/ml; formaldehyde fixation is tolerated) Western Blot (0.5µg/ml for HRPO/ECL detection; recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4,000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.