Alpha Fodrin

Size

£639.00

SKU:: 589942
Purity:: Partially purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Chicken blood cell membranes following hypotonic lysis and mechanical enucleation.
Clone:: AA6
Format:: Supplied as a liquid in PBS, 1% BSA, 0.09% sodium azide.
Extra Details:: Fodrin, also referred to as non-erythroid (alphaII-; brain) spectrin, is a tetrameric (alpha gamma)2 actin-binding, fibrous protein, widely distributed in vertebrates, which forms part of the sub-membranous cytoskeleton within many cell types including neurons, and is particularly abundant with axons. The alpha-subunits of fodrins and spectrin are highly conserved phylogenetically, with the exception of human alpha-fodrin, which shares only 55-59% homology with erythroid-specific alpha-spectrins. The beta-subunits of spectrin (and gamma-subunits of fodrins) are species specific. The interleukin-1 converting enzyme (ICE) family of proteases has been implicated as important effectors of the apoptotic pathway, perhaps acting hierarchically in a protease cascade. Neuronal fodrin is known to be cleaved by calpain following ischaemic insult and it has been proposed that calpain and an unidentified protease play a role in the onset of neuronal death following transient forebrain ischaemia. Recently, an ICE-like protease has been implicated in the early cleavage of fodrin, producing a 150kD fragment, proximal to CPP32 in Fas-induced and C2-ceramide mediated apoptosis. A cleavage product of alpha-fodrin has been proposed as a candidate autoantigen in primary Sjögren’s syndrome and alpha-fodrin has been shown to be the source of a so-called ‘inhibitory protein factor’ family, members of which have been shown to inhibit both GABA and ATP-dependent glutamate uptake into purified synaptic vesicles. Applications: Suitable for use in Western Blot and immunohistochemistry. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice