Akt1, PH Domain

Size

£842.00

SKU:: 589939
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Peptide corresponding to the PH domain of human Akt1
Clone:: RM316
Format:: Supplied as a liquid in PBS, 1% BSA, 0.09% sodium azide, 50% glycerol.
Extra Details:: Akt1, also known as protein kinase Bα (PKBα), is a serine/threonine kinase belonging to the AGC kinase family and one of three Akt isoforms in mammals.1,2 Akt kinases function downstream of activated tyrosine kinases and PI3K to regulate a variety of cellular processes, including cell size, growth, proliferation, and survival, as well as genome stability, glucose metabolism, and neovascularization.2 Akt1 is composed of an N-terminal pleckstrin homology (PH) domain, which binds to phosphatidylinositol-(3,4,5)-triphosphate (PIP3) and phosphatidylinositol-(3,4)-diphosphate (PIP2), a kinase domain, and a C-terminal regulatory hydrophobic motif.3 The Akt PH domain is required for membrane localization and activation of Akt.4 It binds PIP3 and PIP2 generated by PI3K, which is activated by a variety of growth factors, recruiting Akt to the plasma membrane where it is phosphorylated at threonine 308 and serine 473, resulting in its activation.2 A glutamic acid-to-lysine substitution at glutamic acid 17 (E17K) in the Akt1 PH domain leads to constitutive Akt1 membrane localization and activation and has been found in tumors isolated from patients with breast, colorectal, or ovarian cancer.5 Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:500-1:10.000 Immunohistochemistry (FFPE): 1:200-1:500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice