589931
Actin
Size
£537.00
- SKU:
- 589931
- Application:
- WB
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Bovine skeletal muscle Actin.
- Format:
- Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol.
- Extra Details:
- Actin is one of the major proteins (~42kD) of the cytoskeleton. It regulates contractile potential in muscle tissues and helps to control the shape as well as the motility of non-muscle cells. Actin is expressed in all eukaryotic cells which makes it a useful loading control in Western blotting experiments. In higher eukaryotes, there are six isoforms of actin: Three alpha-actins (alpha-skeletal, alpha-cardiac, alpha-smooth muscle), one beta-actin (beta-non-muscle) and two gamma-actins (gamma-smooth muscle and gamma-non-muscle). Actin isoforms show >90% overall sequence homology. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, ECL Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice