ACTH - 2BScientific

SKU:

589930

Application:

IHC-Fr

Purity:

Purified by Protein G affinity chromatography.

Storage Conditions:

-20°C

Supplier:

United States Biological

Host:

Mouse

Immunogen:

N-terminal peptide within aa1-24 from the Synacthen/ACTH region of human POMC.

Clone:

r57 (recomb)

Format:

Supplied as a liquid in PBS, 0.1mg/ml BSA, 0.05% sodium azide.

Extra Details:

ACTH (adrenocorticotropic hormone, corticotropin) is a 39aa active peptide produced by the anterior pituitary. POMC (pro-opiomelanocortin or corticotropin-lipotropin) is a 267aa polypeptide hormone precursor that goes through extensive, tissue-specific posttranslational processing by convertases. POMC is cleaved into ten hormone chains named NPP, ACTH, alpha-MSH (melanocyte stimulating hormone), beta-MSH, gamma-MSH, CLIP (corticotropin-like intermediary peptide), lipotropin-beta, lipotropin-gamma, beta-endorphin and Met-enkephalin. ACTH is also produced by cells of immune system (T-cells, B-cells, and macrophages) in response to stimuli associated with stress. Anti-ACTH is a useful marker in classification of pituitary tumors and the study of pituitary disease. It reacts with ACTH-producing cells (corticotrophs). It also may react with other tumors (e.g. some small cell carcinomas of the lung) causing paraneoplastic syndromes by secreting ACTH. Applications: Suitable for use in Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry (formalin-fixed paraffin embedded): 0.5-1ug/ml for 30 min at RT. Requires HIER. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.