Myosin (Skeletal, Slow)

Size

£850.00

SKU:: 587086
Purity:: Ascites
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Myofibrils isolated from human skeletal muscle
Clone:: NOQ7.5.4D
Format:: Supplied as a liquid, 15mM sodium azide
Extra Details:: Myosin is a 480 kDa protein known to interact with actin in muscle and in non-muscle cells. It contains two identical heavy chains (200 kDa each) and four light chains (15-26 kDa). Myosin 7 (MYH7) is mapped to human chromosome 14q11.1. Applications: Suitable for use in Radioimmunoassay, indirect ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry: (formalin-fixed, paraffin-embedded sections): 1:4000 using protease-digested, sections of rabbit tongue. Detection of slow myosin in formalin-fixed, paraffin-embedded, tissues is greatly enhanced by proteolytic digestion of the preparation. Western Blot: 1:5000 using extract of rat or rabbit tongue Optimal dilutions to be determined by the researcher. Hybridoma: Myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.