Insulin-like Growth Factor, Active, Recombinant, Human, aa52-118 (IGF1)

Size

£314.00

SKU:: 586928
Molecular Weight:: 7.3
Purity:: ≥95% (SDS-PAGE). Endotoxin: ≤0.5EU/ug (LAL).
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a lyophilized powder from 20mM NaAc-HAC, pH 4.5. Reconstitute with sterile ddH2O, to a concentration of 0.1-1mg/ml.
Extra Details:: The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation Partial recombinant protein corresponding to aa52-118 from human Insulin-like growth factor, expressed in E.coli. Accession/Uniprot: P05019 Molecular Weight: ~7.3kD Biological Activity: The ED50 as determined in a serum-free cell proliferation assay using MCF?7 human breast cancer cells is typically 1-8ng/ml. Amino Acid Sequence: TLCGAELVDALQFVCGDRGFYFNKPTGYGSSSRRAPQTGIVDECCFRSCDLRRLEMYCAPLKPAKSA Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice