UV DNA Damage Endonuclease, Recombinant, Bacillus cereus, aa1-317, His-Tag, Myc-Tag

Size

£633.00

SKU:: 586753
Molecular Weight:: 44
Purity:: ≥85% (SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.
Extra Details:: Component in a DNA repair pathway. Removal of UV LIGHT damaged nucleotides. Recognizes pyrimidine dimers and cleave a phosphodiester bond immediately 5' to the lesion. Source: Recombinant protein corresponding to aa1-317 from Bacillus cereus UV DNA damage endonuclease, fused to 10X His-Tag at N-terminal and Myc-Tag at C-terminal, expressed in E.coli. Molecular Weight: ~44.0kD Amino Acid Sequence: MIMRFGYVSHAMALWDCSPAKTMTFTSFKKLSKQEREDKLYHVIRQNLEHTIRILHYNIAHEIPLYRLSSSIVPLATHPEVEFDYIGVFTPLWRKIGALIKEHNLRISFHPNQFTLFTSDKPHITTNAITDMTYHYKILDAIGIADSSYINIHVGGAYGNKEKAIERFHENIKKLPAHIKKQMTLENDDKTYTTSETLSICQKENIPFVFDYHHHMANLCEQPLEELLPAIFETWSHTNISPKVHISSPRSEKEFRAHAEYIDLEFIKPFLHVAKKNNHNFDIMIESKQKDLALFRLIDELSAIRGIKRISGAMLQW Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.