Nucleoside Diphosphate Kinase, Cytosolic, Recombinant, Dictyostelium Discoideum, aa1-155, His-V5-Tag

Size

£633.00

SKU:: 585612
Molecular Weight:: 24.2
Purity:: ≥90% (SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.
Extra Details:: Major role in the synthesis of nucleoside triphosphates other than ATP. Full-length recombinant protein corresponding to aa1-155 from Dictyostelium discoideum Nucleoside diphosphate kinase, cytosolic, fused to 10X His-V5-Tag at N-terminal, expressed in E.coli. Uniprot/Swiss Accession: P22887 Molecular Weight: ~24.2kD Amino Acid Sequence: MSTNKVNKERTFLAVKPDGVARGLVGEIIARYEKKGFVLVGLKQLVPTKDLAESHYAEHKERPFFGGLVSFITSGPVVAMVFEGKGVVASARLMIGVTNPLASAPGSIRGDFGVDVGRNIIHGSDSVESANREIALWFKPEELLTEVKPNPNLYE Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.