Leukotoxin, Recombinant, Mannheimia haemolytica, aa712-954, His-Tag, Myc-Tag

Size

£633.00

SKU:: 585192
Molecular Weight:: 33.9
Purity:: ≥90% (SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.
Extra Details:: Pasteurella leukotoxins are exotoxins that attack host leukocytes and especially polymorphonuclear cells, by causing cell rupture. The leukotoxin binds to the host LFA-1 integrin and induces a signaling cascade leading to many biological effects, including tyrosine phosphorylation of the CD18 tail, elevation of the intracellular Ca(2+) and lysis of the host cell. This leukotoxin is a major contributor to the pathogenesis of lung injury in ovine pneumonic pasteurellosis. It has also weak hemolytic activity. Source: Recombinant protein corresponding to aa712-954 from Mannheimia haemolytica Leukotoxin, fused to 10X His-Tag at N-terminal and Myc-Tag at C-terminal, expressed in E.coli. Molecular Weight: ~33.9kD Amino Acid Sequence: EEIIGTSHNDIFKGSQFNDAFNGGDGVDTIDGNGGNDRLFGGKGDDIIDGGDGDDFIDGGKGNDLLHGGRGDDIFVHRQGDGNDSITEAGGHDRLSFSDSNLKDLTFEKVNHHLVITNTKQEKVTIQNWFREEEFAKTVKNYVATRDEKIEEIIGQNGERITSKQVDELIAKGKDNKIDKNDLANVVNSYELLKNSRNVTNSLDKLISSVSSFTSSNDSRNVLATPTSMLDTSLSSLQFARAA Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice