Glycerol-1-phosphate Dehydrogenase [NAD(P)+], Recombinant, Geobacillus stearothermophilus, aa1-404, His-Tag

Size

£598.00

SKU:: 584706
Molecular Weight:: 47.4
Purity:: ≥90% (SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.
Extra Details:: Catalyzes the NAD(P)H-dependent reduction of dihydroxyacetonephosphate (DHAP or glycerone phosphate) to glycerol 1-phosphate (G1P). The G1P thus generated is probably used for the synthesis of phosphoglycerolipids in Gram-positive bacterial species. Source: Recombinant protein corresponding to aa1-404 from Geobacillus stearothermophilus Glycerol-1-phosphate dehydrogenase [NAD(P)+], fused to 6X His-Tag at N-terminal, expressed in Yeast. Molecular Weight: ~47.4kD Amino Acid Sequence: MNQTLEEVVSLARQCGCGHRHYDIPIEQMVVGREAFARLVAYLRHKRYERVAIVADDHTFAAVERSLCDQLENGSIRYTVCLVQPDENGDVIADERSIVQVLLETPDDVDVLIAVGAGTIHDITRFSSYKMRIPFISVPTAPSVDGFTSMGAPLIIRGVKKTIQAQAPIAVFAHTGVLCQSPKEMIAAGFGDMVAKYTSLADWQFAHWMADEPYCPFVHQLTEQSLQTCVDHIDDIAAGGEQGIRVLMDALLQSGIAMLLMGQSYSASGAEHHLSHYWEMEFLRQKKRQVLHGAKVGVSTPIIIEHYQRVFWPLLNELEKRPKSMDEATWERLKANTASIRELLESLPSPERIRTMLAKVGGAIAPEQLGIDPQLVERSLREAHRLRLNRFTMLYFLNELIFVE Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice