Collagen Alpha-3(IV) Chain, Recombinant, Bovine, aa234-471, His-Tag

Size

£633.00

SKU:: 584046
Molecular Weight:: 30
Purity:: ≥85% (SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.
Extra Details:: Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. Source: Recombinant protein corresponding to aa234-471 from bovine Collagen alpha-3(IV) chain, fused to 6X His-Tag at N-terminal, expressed in E.coli. Molecular Weight: ~30.0kD Amino Acid Sequence: DTGPPAAGAVMRGFVFTRHSQTTAIPSCPEGTEPLYSGFSLLFVQGNEQAHGQDLGTLGSCLQRFTTMPFLFCNINDVCNFASRNDYSYWLSTPAMIPMDMAPITGRALEPYISRCTVCEGPAIAIAVHSQTTDIPPCPAGWISLWKGFSFIMFTSAGSEGAGQALASPGSCLEEFRASPFIECHGRGTCNYYSNSYSFWLASLDPKRMFRKPIPSTVKAGELENIISRCQVCMKMRP Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.