Cell Surface Hyaluronidase, Recombinant, Mouse, aa104-250, His-Tag

Size

£598.00

SKU:: 583961
Molecular Weight:: 18.7
Purity:: ≥85% (SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.
Extra Details:: Cell surface hyaluronidase that mediates the initial cleavage of extracellular high-molecular-weight hyaluronan into intermediate-size hyaluronan of approximately 5kD fragments. Acts as a regulator of angiogenesis and heart morphogenesis by mediating degradation of extracellular hyaluronan, thereby regulating VEGF signaling. Is very specific to hyaluronan; not able to cleave chondroitin sulfate or dermatan sulfate. Source: Recombinant protein corresponding to aa104-250 from mouse Cell surface hyaluronidase, fused to 10X His-Tag at N-terminal, expressed in Yeast. Molecular Weight: ~18.7kD Amino Acid Sequence: SSKYAPDENCPDQNPRLRNWDPGQDSAKHIVIKEGDLFRLTSDATVDSIVIQDGGLLVFGDDKDGSKNITLRTRYILIQDGGALHIGAEKCRYRSKATITLYGKSDERESMPIFGKKFIGVEAGGTLELHGAQRTSWTMLARTLHSS Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.