Bacteriocin Plantaricin-A, Recombinant, Lactobacillus plantarum, aa26-48, His-SUMO-Tag, Myc-Tag

Size

£633.00

SKU:: 583751
Molecular Weight:: 18.7
Purity:: ≥90% (SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.
Extra Details:: This heat stable bacteriocin inhibits the growth of closely related Lactobacillus species. It may act as a pore-forming protein, creating a channel in the cell membrane through a 'barrel stave' mechanism. Source: Recombinant protein corresponding to aa26-48 from Lactobacillus plantarum Bacteriocin plantaricin-A, fused to 10X His-SUMO-Tag at N-terminal and Myc-Tag at C-terminal, expressed in E.coli. Molecular Weight: ~18.7kD Amino Acid Sequence: AYSLQMGATAIKQVKKLFKKWGW Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.