Insulin, Bovine Pancreas

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£326.00

SKU:: 583350
Molecular Weight:: 5.8
Storage Conditions:: -70°C
Supplier:: United States Biological
CAS Number:: 11070-73-8
Extra Details:: Two-chain polypeptide hormone produced by the β-cells of pancreatic islets. Its molecular weight is ~5800D. The α and β chains are joined by two interchain disulfide bonds. The α chain contains an intrachain disulfide bond. Insulin regulates the cellular uptake, utilization, and storage of glucose, amino acids, and fatty acids and inhibits the breakdown of glycogen, protein, and fat. Source: Bovine Pancreas CAS No: 11070-73-8 Molecular Weight: 5733.49 Molecular Formula: C254H377N65O75S6 Purity: ≥97% Appearance: White to off-white solid Solubility: Water (10mg/ml) (ultrasonic and warming, adjust pH to 3 with HCl and heat to 60ºC). Applications: Suitable for use in cell culture applications at 0.5ml/liter of medium. Amino Acid Sequence: Phe-Val-Asn-Gln-His-Leu-Cys-Gly-Ser-His-Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val-Cys-Gly-Gl u-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala. Gly-Ile-Val-Glu-Gln-Cys-Cys-Ala-Ser-Val-Cys- Ser-Leu-Tyr-Gln-Leu-Glu-Asn-Tyr-Cys-Asn (Disulfide bridge: Cys7-Cys7', Cys19-Cys20', Cys6'-Cys11’) Biological Activity: 27u/mg Endotoxin: ≤10EU/mg Loss on Drying: ≤10% Zn Content: As Reported Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -70°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -80°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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