VISTA

Size

£614.00

SKU:: 583327
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Extracellular domain of human VISTA.
Clone:: 9E4
Format:: Supplied as a liquid in PBS, 0.02% sodium azide, 50% glycerol.
Extra Details:: VISTA/B7-H5/platelet receptor Gi24 is a single-pass type I membrane protein located at the cell surface. It is an immunoregulatory receptor which can inhibit T-cell response and may promote differentiation of embryonic stem cells, by inhibiting the BMP4 signaling pathway. The protein can be cleaved by MMP14, and stimulate MMP14-mediated MMP2 activation. Applications: Suitable for use in ELISA, Flow Cytometry, Immunocytochemistry, Immunofluorescence and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry (paraffin): 2ug/ml Immunocytochemistry: 1ug/ml Immunofluorescence: 2ug/ml Flow Cytometry: 10ug/ml Optimal dilutions to be determined by the researcher. Positive Control: Human Lymphoma Tissue Lysate, Human Spleen Tissue Molecular Weight: ~31kD (predicted), 47kD (observed) Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.