TIGIT

Size

£614.00

SKU:: 583302
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Extracellular domain of human TIGIT.
Clone:: 10B1
Format:: Supplied as a liquid in PBS, 0.02% sodium azide, 50% glycerol.
Extra Details:: The T cell immunoreceptor with Ig and ITIM domains (TIGIT) is a member of the PVR (poliovirus receptor) family of immunoglobin proteins. It is expressed on several classes of T cells including follicular B helper T cells (TFH). TIGIT has been shown to bind PVR with high affinity; this binding is thought to assist interactions between TFH and dendritic cells to regulate T cell dependent B cell responses (1). Similar to other immune checkpoint proteins such as PD-1, TIGIT is upregulated on exhausted T cells in chronic viral infections and cancer. Blockade of both TIGIT and PD-1 pathways leads to tumor rejection in mice suggesting that it may be of therapeutic use against cancer (2). Applications: Suitable for use in ELISA, Flow Cytometry, Immunocytochemistry, Immunofluorescence and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry (paraffin): 2ug/ml Immunofluorescence: 1ug/ml Flow Cytometry: 1ug/ml Immunocytochemistry: 1ug/ml Optimal dilutions to be determined by the researcher. Positive Control: Human Stomach Carcinoma Tissue Lysate Molecular Weight: ~26kD (predicted), 47kD (observed) Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice