583301
TIGIT
Size
£614.00
- SKU:
- 583301
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Extracellular domain of human TIGIT.
- Clone:
- 4A12
- Format:
- Supplied as a liquid in PBS, 0.02% sodium azide, 50% glycerol.
- Extra Details:
- The T cell immunoreceptor with Ig and ITIM domains (TIGIT) is a member of the PVR (poliovirus receptor) family of immunoglobin proteins. It is expressed on several classes of T cells including follicular B helper T cells (TFH). TIGIT has been shown to bind PVR with high affinity; this binding is thought to assist interactions between TFH and dendritic cells to regulate T cell dependent B cell responses (1). Similar to other immune checkpoint proteins such as PD-1, TIGIT is upregulated on exhausted T cells in chronic viral infections and cancer. Blockade of both TIGIT and PD-1 pathways leads to tumor rejection in mice suggesting that it may be of therapeutic use against cancer (2). Applications: Suitable for use in ELISA, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilution: Immunohistochemistry (paraffin): 2ug/ml Immunofluorescence: 1ug/ml Flow Cytometry: 1ug/ml Immunocytochemistry: 1ug/ml Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher. Positive Control: Human Stomach Carcinoma Tissue Lysate Molecular Weight: ~26kD (predicted), 47kD (observed) Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice