583221
PD1
Size
£614.00
- SKU:
- 583221
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Extracellular domain of human PD-1.
- Clone:
- 5D3
- Format:
- Supplied as a liquid in PBS, 0.02% sodium azide, 50% glycerol.
- Extra Details:
- Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antigen-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD-1-null mice who exhibit hyperactivated immune systems and autoimmune diseases. PD-1 is thus one of a growing number of immune checkpoint proteins. Applications: Suitable for use in ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunofluorescence and Western Blot. Other applications not tested. Recommended Dilution: Immunohistochemistry (paraffin): 5ug/ml Immunofluorescence: 20ug/ml Flow Cytometry: 1ug/ml Optimal dilutions to be determined by the researcher. Positive Control: Human Spleen Tissue Lysate, Human Tonsil Tissue Lysate, Human Lymph node Tissue Lysate, Human Spleen Tissue Slide Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice