583166
CD86
Size
£655.00
- SKU:
- 583166
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to 17 amino acid near the center of human CD86. Species sequence homology: rabbit: (75%) and mouse: (75%)
- Format:
- Supplied as a liquid in PBS, 0.02% sodium azide.
- Extra Details:
- CD86, also known as B7-2, is a type I membrane protein that is a member of the immunoglobulin superfamily. Like the related protein CD80, this protein is expressed by antigen-presenting cells, and is the ligand for two proteins at the cell surface of T cells, CD28 and the cytotoxic T-lymphocyte-associated protein 4 (CTLA-4). Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell and induces T-cell proliferation and cytokine production. CTLA-4 binding negatively regulates T-cell activation and diminishes the immune response (1). Blocking the CTLA-4-CD80/CD86 interaction has been shown to enhance T-cell functions in acute lymphoblastomic leukemia (ALL), suggesting that this pathway may be an attractive target for future cancer immunotherapy (2). Applications: Suitable for use in ELISA, Immunohistochemistry, Immunofluorescence and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1-2ug/ml Immunohistochemistry (paraffin): 2ug/ml Immunofluorescence: 20ug/ml Optimal dilutions to be determined by the researcher. Positive Control: Daudi Cell Lysate, Raji Cell Lysate Molecular Weight: ~36kD (predicted), 68kD (observed) Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice