CD80

Size

£614.00

SKU:: 583161
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Extracellular domain of human CD80.
Clone:: 10A1
Format:: Supplied as a liquid in PBS, 0.02% sodium azide, 50% glycerol.
Extra Details:: CD80, also known as B7-1, is a type I membrane protein that is a member of the immunoglobulin superfamily. Like the related protein CD86, this protein is expressed by antigen-presenting cells, and is the ligand for two proteins at the cell surface of T cells, CD28 and the cytotoxic T-lymphocyte-associated protein 4 (CTLA-4). Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell and induces T-cell proliferation and cytokine production. CTLA-4 binding negatively regulates T-cell activation and diminishes the immune response (1). Blocking the CTLA-4-CD80/CD86 interaction has been shown to enhance T-cell functions in acute lymphoblastomic leukemia (ALL), suggesting that this pathway may be an attractive target for future cancer immunotherapy (2). Applications: Suitable for use in ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry and Flow Cytometry. Other applications not tested. Recommended Dilution: Immunohistochemistry (paraffin): 2-5ug/ml Flow Cytometry: 10ug/ml Immunofluorescence: 5ug/ml Optimal dilutions to be determined by the researcher. Positive Control: Human Stomach Carcinoma Tissue Lysate, Human Tonsil Tissue Molecular Weight: ~32kD (predicted), 50kD (observed) Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice