553810
IgE
Size
£698.00
- SKU:
- 553810
- Purity:
- Purified by Protein G affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to sheep IgE1-2 expressed in E. coli.
- Clone:
- 1E7
- Format:
- Supplied as a liquid in PBS, 0.09% sodium azide.
- Extra Details:
- IgE is an immunoglobulin primarily produced from plasma cells and, in normal serum, present at very low concentrations. Western blot analysis against 553810 demonstrates a single major band of approximately 80kD under reducing conditions, and a band at approximately 200kD is observed after electrophoresis under non-reducing conditions. These bands correspond with the expected molecular weights of the epsilon chain and the complete ovine IgE molecule (Kooyman, F.N. et al. 1997). IgE is in important in both type 1 hypersensitivity and immunity to parasite infections, in particular parasitic worm infections. 553810 has been used in a number of studies involving parasite nematode infections of sheep by Haemonchus contortus (Vervelde, L. et al. 1997 and Kooyman, F.N. et al. 1997). This antibody has also been reported to recognize bovine IgE, and, as such, it is useful for studies of infection in cattle by nematode worms such as Dictyocaulus viviparus (Bricarello, P.A. et al. 2007 and Kooyman, F.N. et al. 2002). Applications: Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:500-1:2000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice