CD178 (Fas Ligand) (Preservative Free, Low Endotoxin)

Size

£488.00

SKU:: 553698
Application:: Flow Cytometry
Purity:: Purified by affinity chromatography. Endotoxin: ≤0.01EU/ug (LAL).
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Hamster
Immunogen:: B6 mouse FasL cDNA-transfected baby hamster kidney (B6 FasL/BHK) cells
Clone:: MFL3
Format:: Supplied as a liquid in PBS, pH 7.2. No preservative added.
Extra Details:: CD178 is a 40 kD member of the TNF/NGF superfamily also known as Fas ligand, FasL, Apo-1 ligand, and CD95 ligand. Cell surface CD178 is expressed on activated T cells and in testis and eye. CD178 is upregulated in activated T cells upon TCR re-engagement and has been shown to induce autocrine and paracrine T cell death. CD178 expression in the eye and testis has been shown to participate in immune privilege at these sites. CD178 can be cleaved from the surface by metalloproteases, and "soluble" CD178 may block the activities of membrane-bound CD178. CD178 binds to CD95 (Fas) to induce apoptotic cell death implicated in the maintenance of peripheral tolerance. CD178/CD95 interactions have also been implicated in the proliferation of CD8+ cells and neutrophil extravasation, chemotaxis, and survival. The MFL3 antibody recognizes CD178 in a wide array of mouse strains and has been reported to block CD178/CD95 induced apoptosis. Applications: Suitable for use in Flow Cytometry. This antibody has also been reported in blocking andI mmunocytochemistry.. Other applications not tested. Recommended Dilution: Flow Cytometry: ≤ 0.25ug/10e6 cells in 100ul Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice