CD43

Size

£652.00

SKU:: 552776
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Purified recombinant protein fragment corresponding to aa: extra 20-169 from human CD43, expressed in E. coli.
Clone:: 3E12F3
Format:: Supplied as a liquid in PBS, 0.05% sodium azide.
Extra Details:: This gene encodes a highly sialylated glycoprotein that functions in antigen-specific activation of T cells and is found on the surface of thymocytes, T lymphocytes, monocytes, granulocytes and some B lymphocytes. It contains a mucin-like extracellular domain, a transmembrane region and a C-terminal intracellular region. The extracellular domain has a high proportion of serine and threonine residues, allowing extensive O-glycosylation and has one potential N-glycosylation site, while the C-terminal region has potential phosphorylation sites that may mediate transduction of activation signals. Different glycoforms of this protein have been described. In stimulated immune cells, proteolytic cleavage of the extracellular domain occurs in some cell types, releasing a soluble extracellular fragment. Defects in expression of this gene are associated with Wiskott-Aldrich syndrome. Applications: Suitable for use in ELISA, Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:10,000 Flow Cytometry: 1:200-1:400 Western Blot: 1:500-1:2000 Immunohistochemistry: 1:200-1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice