CD10 - 2BScientific

SKU:

552548

Molecular Weight:

0

Purity:

Purified

Storage Conditions:

-20°C

Supplier:

United States Biological

Host:

Mouse

Immunogen:

Purified recombinant protein fragment corresponding to aa549-750 from human CD10, expressed in E. coli..

Clone:

6G12E12

Format:

Supplied as a liquid in PBS, 0.05% sodium azide.

Extra Details:

This gene encodes a common acute lymphocytic leukemia antigen that is an important cell surface marker in the diagnosis of human acute lymphocytic leukemia (ALL). This protein is present on leukemic cells of pre-B phenotype which represent 85% of cases of ALL. This protein is not restricted to leukemic cells however and is found on a variety of normal tissues. It is a glycoprotein that is particularly abundant in kidney where it is present on the brush border of proximal tubules and on glomerular epithelium. The protein is a neutral endopeptidase that cleaves peptides at the amino side of hydrophobic residues and inactivates several peptide hormones including glucagon, enkephalins, substance P, neurotensin, oxytocin and bradykinin. This gene, which encodes a 100kD type II transmembrane glycoprotein exists in a single copy of greater than 45kb. The 5' untranslated region of this gene is alternatively spliced resulting in four separate mRNA transcripts. The coding region is not affected by alternative splicing. Applications: Suitable for use in ELISA, Flow Cytometry and Western Blot . Other applications not tested. Recommended Dilution: ELISA: 1:10,000 Flow Cytometry: 1:200-1:400 Western Blot: 1:500-1:2000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.