ACTH

Size

£652.00

SKU:: 552391
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Purified recombinant protein fragment corresponding to human ACTH, expressed in E. coli.
Clone:: 1A9C6
Format:: Supplied as a liquid in PBS, 0.05% sodium azide.
Extra Details:: This gene encodes a preproprotein that undergoes extensive and tissue-specific post-translational processing via cleavage by subtilisin-like enzymes known as prohormone convertases. There are eight potential cleavage sites within the preproprotein and depending on tissue type and the available convertases, processing may yield as many as ten biologically active peptides involved in diverse cellular functions. The encoded protein is synthesized mainly in corticotroph cells of the anterior pituitary where four cleavage sites are used; adrenocorticotrophin, essential for normal steroidogenesis and the maintenance of normal adrenal weight and lipotropin beta are the major end products. In other tissues, including the hypothalamus, placenta and epithelium, all cleavage sites may be used giving rise to peptides with roles in pain and energy homeostasis, melanocyte stimulation and immune modulation. These include several distinct melanotropins, lipotropins and endorphins that are contained within the adrenocorticotrophin and beta-lipotropin peptides. The antimicrobial melanotropin alpha peptide exhibits antibacterial and antifungal activity. Mutations in this gene have been associated with early onset obesity, adrenal insufficiency and red hair pigmentation. Alternatively spliced transcript variants encoding the same protein have been described Applications: Suitable for use in ELISA, Flow Cytometry and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:10,000 Flow Cytometry: 1:200-1:400 Western Blot: 1:500-1:2000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice