Fibroblast Growth Factor Receptor 1 (FGFR1, Basic fibroblast growth factor receptor 1, BFGFR, bFGF-R, CD331, CEK, c-fgr, FGFBR, FGFR-1, FLG, FLJ99988, FLT2, Fms-like tyrosine kinase 2, H2, H3, H4, H5, HBGFR, KAL2, N-SAM, OGD)

Size

£659.00

SKU:: 552381
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to the C-terminus of human FGF receptor 1.
Clone:: D8E4
Format:: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.
Extra Details:: Fibroblast growth factors (FGFs) produce mitogenic and angiogenic effects in target cells by signaling through the cellular surface tyrosine kinase receptors. There are four members of the FGF receptor family: FGFR-1 (flg), FGFR-2 (bek, KGFR), FGFR-3 and FGFR-4. Each receptor contains an extracellular ligand binding domain, a transmembrane region and a cytoplasmic kinase domain (1). Following ligand binding and dimerization, the receptors are phosphorylated at specific tyrosine residues (2). Seven tyrosine residues in the cytoplasmic tail of FGFR-1 can be phosphorylated: Tyr463, Tyr583, Tyr585, Tyr653, Tyr654, Tyr730 and Tyr766. Tyrosine 653 and 654 are important for catalytic activity of the activated FGFR and are essential for signaling (3). The other phosphorylated tyrosine residues may provide docking sites for downstream signaling components such as Crk and PLCgamma (4,5). Applications: Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Flow Cytometry and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking overnight. Immunoprecipitation: 1:50 Immunohistochemistry (paraffin): 1:500 Immunofluorescence (IF/IC): 1:200 Flow Cytometry: 1:400 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice