TNFRSF11A, Recombinant, Mouse, aa31-214, hIgG-His-Tag

Size

£409.00

SKU:: 552330
Molecular Weight:: 47.5
Purity:: ≥90% (SDS-PAGE). Endotoxin: ≤1EU/ug (LAL).
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in PBS, pH 7.4, 10% glycerol
Extra Details:: RANK, as known as Tumor necrosis factor receptor superfamily member 11A (TNFRSF11A) is a member of the tumor necrosis factor receptor (TNFR) molecular sub-family. It is the receptor for RANK-Ligand (RANKL) and part of the RANK/RANKL/OPG signaling pathway that regulates osteoclast differentiation and activation. It is associated with bone remodeling and repair, activation of NF-kappa B and c-jun N-terminal kinase, enhancement of T cell growth and dendritic cell function, immune cell function, lymph node development, thermal regulation, and mammary gland development. It is able to block TRANCE induced biological activity. Recombinant mouse RANK, fused to hlgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques. Recombinant protein corresponding to aa31-214 from mouse RANK/TNFRSF11A, fused to hIgG-His-Tag at C-terminal, expressed in insect cell (Baculovirus). Accession/Uniprot: O35305 Molecular Weight: ~47.5kD (426aa) Amino Acid Sequence: <ADL>VTPPCTQ ERHYEHLGRC CSRCEPGKYL SSKCTPTSDS VCLPCGPDEY LDTWNEEDKC LLHKVCDAGK ALVAVDPGNH TAPRRCACTA GYHWNSDCEC CRRNTECAPG FGAQHPLQLN KDTVCTPCLL GFFSDVFSST DKCKPWTNCT LLGKLEAHQG TTESDVVCSS SMTLRRPPKE AQAYLPS<LEP KSCDKTHTCP PCPAPELLGG PSVFLFPPKP KDTLMISRTP EVTCVVVDVS HEDPEVKFNW YVDGVEVHNA KTKPREEQYN STYRVVSVLT VLHQDWLNGK EYKCKVSNKA LPAPIEKTIS KAKGQPREPQ VYTLPPSRDE LTKNQVSLTC LVKGFYPSDI AVEWESNGQP ENNYKTTPPV LDSDGSFFLY SKLTVDKSRW QQGNVFSCSV MHEALHNHYT QKSLSLSPGK HHHHHH> Biological Activity: Measured by its binding ability in a functional ELISA with mouse RANKL/TNFSF11 (Catalog # 532392). The ED50 range ≤ 200ng/ml. Applications: Suitable for use in Bioactivity and SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice