TF-1 Lysate

Size

£363.00

SKU:: 552322
Storage Conditions:: -70°C
Supplier:: United States Biological
Format:: Supplied as a SDS-PAGE Sample Buffer (0.12M Tris-HCl buffer pH6.8, 0.4% SDS, 5% glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue)
Extra Details:: TF-1 cells have been derived from a patient with erythroleukaemia. TF-1 cells are completely dependent on GM-CSF or IL-3 for long term growth. TF-1 cells respond to a variety of other lymphokines and cytokines such as IL-1, IL-4, IL-6, IL-9, IL-11, IL-13, SCF, LIF and NGF. TF-1 cells do not express glycophorin A or carbonyl anhydrase I. Quality Control: Western Blot analyzed by both GAPDH and Beta tubulin antibody Preparation Before Usage: Heat lysate to 95°C for 5 minutes and rapidly cool before use. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.