552028
IL1RA, Recombinant, Human, aa26-177
Size
£351.00
- SKU:
- 552028
- Molecular Weight:
- 17.2
- Purity:
- ≥95% (SDS-PAGE). Endotoxin: <1EU/1ug (LAL)
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Format:
- Supplied as a liquid in PBS, pH 7.4.
- Extra Details:
- IL-1RA/IL-1F3, also known as interleukin-1 receptor antagonist, is a glycosylated protein and a powerful antiinflammatory agent. IL‐1RA is a pan‐physiological inhibitor of IL‐1 that prevents the activation of IL‐1 receptors by inhibiting IL‐1 binding to its receptors. It can inhibit angiogenesis by blocking the activation of the IL‐1/PI3K/NF‐kappaB pathway in IL‐1‐producing human colon cancer cells. Also, Several autoimmune and inflammatory diseases have been associated with an allelic polymorphism of the IL-1RA. Recombinant human IL1RA was expressed in E. coli and purified by using conventional chromatography techniques. Source: Recombinant protein corresponding to aa26-177 from human IL1RA, expressed in E. coli. AA Sequence: MRPSGRKSSK MQAFRIWDVN QKTFYLRNNQ LVAGYLQGPN VNLEEKIDVV PIEPHALFLG IHGGKMCLSC VKSGDETRLQ LEAVNITDLS ENRKQDKRFA FIRSDSGPTT SFESAACPGW FLCTAMEADQ PVSLTNMPDE GVMVTKFYFQ EDE Biological Activity: The activity is determined by the IFN-g ELISA in a using NK-92 human natural killer cells. The ED50 range ≤10ng/ml. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice