551978
GM-CSF, Recombinant, Porcine, aa18-144, His-Tag
Size
£655.00
- SKU:
- 551978
- Molecular Weight:
- 16.6
- Purity:
- ≥95% (SDS-PAGE). Endotoxin: <1EU/1ug (LAL)
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Format:
- Supplied as a liquid in PBS, pH 7.4.
- Extra Details:
- GM-CSF, also known as granulocyte macrophage colony-stimulating factor, is a hematopoietic growth factor that stimulates the development of neutrophils and macrophages and promotes the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells. It is produced in by endothelial cells, monocytes, fibroblasts and T-lymphocytes. GM-CSF inhibits neutrophil migration and enhances the functional activity of the mature end-cells. Recombinant porcine GM-CSF was expressed in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer. Recombinant protein corresponding to aa18-144 from porcine GM-CSF, fused to His-Tag expressed in E. coli. Amino Acid Sequence: <MGSSHHHHHH SSGLVPRGSH M>APTRPPSPV TRPWQHVDAI KEALSLLNNS NDTAAVMNET VDVVCEMFDP QEPTCVQTRL NLYKQGLRGS LTRLKSPLTL LAKHYEQHCP LTEETSCETQ SITFKSFKDS LNKFLFTIPF DCWGPVKK Biological Activity: Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 range ≤40ng/ml. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice