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551860

C3H/101/2 Lysate

Size

£363.00

SKU:
551860
Storage Conditions:
-70°C
Supplier:
United States Biological
Format:
Supplied as a SDS-PAGE Sample Buffer (0.12M Tris-HCl buffer pH6.8, 0.4% SDS, 5% glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue)
Extra Details:
C3H/10T1/2 cell line was isolated in 1972 from a line of C3H mouse embryo cells. The cells are contact sensitive and there is no detectable background spontaneous transformation. They are highly susceptible to transformation by chemical agents and express H-2k antigen. Tested and found negative for ectromelia virus. Quality Control: Western Blot analyzed by both GAPDH and Beta tubulin antibody Preparation Before Usage: Heat lysate to 95°C for 5 minutes and rapidly cool before use. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Shipping Conditions:
Dry Ice