BaF3 Lysate

Size

£363.00

SKU:: 551849
Storage Conditions:: -70°C
Supplier:: United States Biological
Format:: Supplied as a SDS-PAGE Sample Buffer (0.12M Tris-HCl buffer pH6.8, 0.4% SDS, 5% glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue)
Extra Details:: BaF3 cell line was immortalized murine bone marrow-derived pro-B-cell line. BaF3 cells are depends IL3 for growth and proliferation. When cultured in the presence of IL3, or other growth-promoting factors, BaF3 cells are highly resistant to X-irradiation and the cytotoxic drugs. Quality Control: Western Blot analyzed by both GAPDH and Beta tubulin antibody Preparation Before Usage: Heat lysate to 95°C for 5 minutes and rapidly cool before use. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.